"He is not fit to be mayor of our great city. He is not fit to hold any public office. A man who lacks character makes a mockery of his ideas," she said.
Jackson said Filner "refused to listen to someone he had known for 35 years, and who told him explicitly, during a senior staff meeting, that his behavior with women was terrible and possibly illegal."
Filner "laughed it off," she said. She said Filner challenged her to provide one example of improper behavior; when she brought up his comments about wearing underwear, "he had no comeback," she said.
• Laura Fink
After hearing of Jackson's lawsuit, political consultant Laura Fink came forward with her accusations against Filner on Tuesday, saying the then-congressman patted her "posterior" at a fundraising dinner in 2005 when she working as the deputy manager of Filner's congressional campaign.
Fink said she didn't go public with the incident at the time because she was trying to build her political career.
The alleged sexual harassment occurred at the fundraiser, where Fink guided Filner from table to table.
One guest told Filner that Fink "had worked her tokus off," Fink said.
"The congressman told me to turn around and patted me on the bum and said, 'Nope, it's still there' and laughed," Fink told CNN.
Fink wrote to Filner's chief of staff and demanded that Filner apologize, she said.
Filner responded, and "he mumbled an apology" and added something "I just did not understand," Fink said.
On Wednesday, Fink told CNN that Filner should resign.
• Morgan Rose
Nonprofit leader Morgan Rose told CNN on Wednesday that she recently told authorities about her experience with Filner after hearing similar accusations against the mayor in recent weeks. She called a hotline set up by the San Diego Sheriff's Department for accusers who are allowed to remain anonymous under California law.
Rose said she met Filner in April 2009 when he was a congressman to lobby on behalf of her nonprofit organization that focuses on domestic violence.
The two met at a restaurant near Filner's congressional office in Chula Vista, California, she told CNN.
At that meeting, she said, Filner allegedly looked her up and down, stared into her eyes and said, "Your eyes have bewitched me," Rose said.
Filner allegedly then made a remark along the lines of, according to Rose's paraphrasing, "You'll have to excuse me for what's about to happen. It's your fault," Rose said.
Filner then moved to her side of the booth, sat beside her, pinned her to the wall, and put his arm around her, Rose alleged.
Rose avoided the advances, and Filner was suddenly interrupted by his ringing cell phone, Rose said. Filner then left the restaurant, she said.