Pipeline and Hazardous Materials Safety Administration, the Michigan attorney general's office and the state Department of Environment, Great Lakes and Energy.
A state judge granted a request from Nessel to close the line June 25 after Enbridge reported the damage.
The Enbridge report said the nature of the damage and drag marks in the lakebed pointed to a cable or anchor from a small or moderately sized vessel.
Enbridge says it has stepped up security measures in the straits since a 6-ton (5.44-metric tonne) barge and tugboat anchor struck Line 5 in April 2018.
They have focused primarily on avoiding contact with larger vessels but are being expanded to prevent encounters with smaller ones, Duffy said.